Mario and Co arrive in Cramalot, only to be assaulted by Beezos. They are saved by Mervin the magician, who tells them about how Koopa took over Cramalot, and how he can be defeated with a golden plunger that one must pull from a golden sink. Although Mario succeeds in pulling the plunger, he ends up losing it to Koopa, and the group is sentenced to execution in a dungeon.
Mario and Co are traveling through a desert, dying of thirst. Thanks to a booby-trap, they wind up in the palace of Sultan Pasbah, who tries to force Princess into his harem, while the guys are sent to the dungeon. Through total fate, the Marios discover a magic lamp. However, it contains a grouchy genie who apparently offers no help in getting Princess back from the sultan, or from Koopa, who just showed up to purchase her.
The Marios and Toad are trying to save the Princess from Koopa's riverboat. When their plan goes horribly wrong, Toad gets caught and the Marios barely escape with their lives. The Marios then meet up with Mark Twang, a man who's in a race with Koopa. The Marios and Mark now have to work together to beat Koopa and rescue Princess Toadstool and Toad.
Desperate for cash, the Marios trade the royal cow for bean seeds. Princess scolds them, and accidentally sneezes, blowing the beans into the yard. Overnight, they grow into a beanstalk. The next morning, Mario and Co climb the beanstalk, which takes them to a gigantic castle in the sky, which is occupied by Koopa, who's now giant-sized.
Mario and Co arrive in Robo Land, only to be attacked by Koopa, who's donned a robo suit. They meet up with Bunsen, a robot Mushroom, who temporarily gets rid of Koopa. Bunsen then takes the group to Dr. Nerdnick, the man who was tricked into building the robo suit, and is being held captive by Koopa's guards. Everything goes well, until Koopa returns and captures Princess and Toad.
Mario and Co meet Hercufleas, a legendary hero and a friend of Toad's who recently became incredibly fat and lazy. Hercufleas' job is guarding The Great Balls of Fire, which are soon stolen by some Beezos. When the Marios fail to get them back, they turn to Hercufleas. But first, they have to get the big guy back into shape.
Mario and Co arrive in the Oriental city of Sayonara, looking for Super Sushi, an experimental food that can double one's size and strength. They also find Koopa, who's now gigantic, and destroying the city. Mario and Co meet up with the creator of Super Sushi, who explains that Koopa ingested too much, and needs to be stopped.
Mario and Co arrive in Victoria, hoping to find the great dectective, Herlock Solmes. When they find out that Solmes is missing, Mario dons the name Sherlock Mario, and sets out to find him. Turns out Solmes was kidnapped by Koopa and his gang. Koopa then goes after the Retro Reuter, a weapon that clogs drains, to flood the city with sewer water.
Koopa steals a pot of gold coins belonging to a leprechaun named Murphy. Without his pot of gold coins, Murphy is a jinx who's luck is so bad, that Shamrock Land will flood if he doesn't get his coins back. Mario and Co agree to retrieve the coins, and almost succeed, but Murphy's bad luck results in them being caught.
Koopa has turned all the mushroom people into rocks and has captured Mario & Luigi. He is about to kill them, but spares them in exchange for Princess' hand in marriage. She agrees, but only if he releases the mushroom people as well. Shortly after, Koopa turns back on his word and reverts the mushroom people back into rocks. Now the Mario Bros. have to find a way to stop the wedding.
When a sea monster attacks the town of Happy Harbor, Koopa offers to stop the monster if the citizens make him their king. Mario and Co work with the fearless Captain Abidab to get rid of the monster themselves, but Mario, Luigi and Princess get swallowed by the monster, which is actually a submarine of Koopa's.
While travelling through Jungle Land, Mario is seperated from his friends thanks to Koopa and an Albatoss. Mario then suffers from amnesia, and is taken in by an ape couple, who change his name to Marzan and teach him the ways of the jungle. Meanwhile, Luigi, Princess and Toad have to find Mario and knock some sense into him.
Plumbers Academy Mario loses his golden plunger after a battle with Koopa. He tells Princess & Toad that this was a special plunger because it was given to him by the President of the United States. In pre-show flashback mode, Mario tells how he and Luigi became plumbers, and how they had to put up with their overly strict boss, Sgt. Kooperman (who sounds a lot like Koopa). Kooperman ended up flunking the Marios, who soon found themselves facing their biggest plumbing challenge ever.
Mario and Co are on their way to protect a Mushroom space colony, only to be caught by Darth Koopa's ship, the Koop Star. They fight off some Stormtroopas, followed by Koopa himself, but Koopa ends up freezing them. After having them thawed out, he gives Princess two options: Tell the colonists to surrender, or have their planet destroyed with his Birdo Ray. When Princess refuses to give up, the Birdo Ray is set to fire in thirty minutes, and Mario and Co get dropped into the Koop Star's trash compactor.
It's wintertime, and Mario and Co are on their way through the woods to visit Princess' sick grandmother. Before they entered the woods, Koopa stole everyone's lumber, casing them to all freeze. While Mario, Luigi and Toad stay behind to defrost the villagers, Princess continues to her gramdmother's house alone, where she is pursued not only by Koopa, but by the big bad wolf as well.
Koopa's kidnapped Princess, and Mario, Luigi, and Toad try to rescue her, failing numerous times. Mario realizes that Koopa's ego is his weakspot, so they use a Trojan Koopa (instead of a horse) to break into his fortress. However, it all doesn't go as planned, and they find themselves surrounded by Koopa's guards.
A "slime ghost" is in Mario Bros Plumbing, so Mario contacts Ernie Hudson from Slime Busters. Episode based on Ghostbusters. The ghost takes control of Luigi's body, forcing Ernie to battle him.
Basketball star Magic Johnson guest stars and shows Mario and Luigi some magic tricks.
Mario and Luigi are visited by their neice, Marilyn. The two are surprised at how big she's gotten, but Marilyn's uninvited friends and the party she hosts prove to be an even bigger surprise.
A cooking mishap turns Luigi into a zombie. Elvira guest stars as herself.
Mario must help Elvira deal with a mummy.
When Little Robert (Willard Pugh) comes over, the Mario Bros. attempt to get him to sing on tape.
Mad Donna's car breaks down and asks the Mario Brothers to use their phone. However, Mario places a magic love apple on the table and when Mad Donna eats it she falls in love with Mario.
After a dream about their grandmother Mario calls in a psychic (Elaine Kogan) to try to communicate with the deceased Grandma Mario.
Mario finds a treasure map in their apartment, so the brothers ask Inspector Gadget (Maurice LaMarche) for help searching for the treasure.
When a pizza delivery lady says that she adores a man who can "eat his weight in pizza" Mario attempts to do just that. Eve Plumb guest-stars.
When Little Robert comes over, the Mario Bros. attempt to get him to sing on tape. (The live-action segment in this episode is a repeat of "Tutti Frutti, Oh Mario.")
While fixing their furnace, the Mario Bros. are visited by an old man named Fred Van Winkle. Apparently Fred has been asleep for the last 60 years. Mario and Luigi decide to introduce him to the wonders of modern life.