Jay takes on an exciting challenge as he outfits a wrestler with a ferocious reptile for an upcoming match; Andrea's significant other takes his first trip to the zoo; Darth Gator, a beloved alligator, tries out a new fitness routine.
Jay assigns his daughter, Andrea, to represent the zoo at Repticon; Laura and Juliette surprise Jay with a much-needed reptile ride makeover; Jay brings his scaled and slimy friends to entertain at a kid's birthday party.
Jay sells a one-of-a-kind, highly priced reticulated python morph to a long-time international customer; then Jay accompanies Juliette and Laura to check out potential new locations, as it becomes necessary to expand the zoo.
Laura helps Juliette find the perfect breeding mate for a lizard named Gillie; struggling to be everywhere all at once, Jay attempts an audio tour for his customers; Andrea steps up to the plate to give her first tour.
The zoo gets its first billboard, and Laura must quickly arrange a photo shoot, which means wrangling all the animals, as well as Jay; Juliette and Michael bring Vern the chameleon to model for an art class, where Michael gets a surprise of his own.
Jay meets with a contractor who forces him to rethink the zoo's expansion; the vet drops by to check in on Vita, the red-tailed boa; Juliette retires Spot the monitor lizard; Jay shocks everyone by revealing his plans for the future of the zoo.
Jay puts the local fire department's reptile bravery to the test, and then is challenged in return to step up and face one of his own fears. After Michael shares a newfound connection to the reptiles that he discovered on his recent travels, Juliette brings in a professional to help her evaluate his reptile reading skills.
The Reptile Zoo gets a visit from the world-famous Harlem Globetrotters, who want to team up with Jay and his all-star reptiles for an upcoming show. While Jay and the Globetrotters toss around ideas, Michael nails reptile manicures, and Poppy, the panther chameleon, gets ready to make her debut. The Reptile Zoo's all-star team and the Harlem Globetrotters take the court together to deliver a performance that's a slam dunk.
Jay, Juliette, and the team plan a reptile birthday party surprise for the moms of social media influencers Liane V. and Don Benjamin. Michael gets the chance to run the Reptile Zoo's popular day camp for the first time. Jay's co-author stops by to meet the scaly friends who will be the stars of their new children's book.
The Zoo hosts a vow renewal ceremony for a reptile-loving couple, where some cold-blooded guests make the heart-warming day extra special for friends and family. Social media superstar Brent Rivera enlists the help of Jay and his snakes to play an epic prank on some new members of his crew. Coconut, the albino alligator, celebrates her sixth birthday.
Comedian Gabriel "Fluffy" Iglesias stops by the zoo and quickly learns that Jay's snakes are nothing to laugh at. Jay and Juliette take off on a reptile-filled Florida adventure. First on the agenda is a visit to their friend Sam and his giant tortoises to fulfill a Brewer family tradition. Then it's on to a herp-hunting adventure in the Everglades with herpetologist Edward Bay.
Jay and Juliette wrap up their Florida adventure with a visit to a motion capture studio, where reptile superstars inspire Jay to make a welcome video for the zoo. Back in California, Jay and his team plan an epic bash to celebrate the Reptile Zoo's 15th anniversary and give back to the community. On the day of the big party, Jay celebrates his amazing reptile family, reads from his new children's book, and gives his baby grandson a very special gift.