The Round of 32 begins with the Clubs and Spades brackets, as Humberto Brenes challenges Phil Laak in the feature matchup. On the outer tables are Huck Seed vs. Chip Reese, Barry Greenstein vs. Vanessa Rousso, Shannon Elizabeth vs. Jeff Madsen, Nam Le vs. Annie Duke, Shawn Sheikhan vs. defending champion Ted Forrest, Paul Wasicka vs. Joe Hachem, and Tuan Le vs. T.J. Cloutier.
| Name | Type | Role | |
|---|---|---|---|
| Oliver 'Ali' Nejad | Host | Himself - Commentator |