After a naive real estate agent succumbs to the will of Count Dracula, the two head to London where the vampire sleeps in his coffin by day and searches for potential victims by night.
El conde Drácula (Carlos Villarías) viaja a Londres y se instala en su recién adquirida abadía de Carfax, junto al sanatorio del doctor Seward. Allí, el conde se encapricha con Eva (Lupita Tovar) y Van Helsing (Eduardo Arozamena), mientras tanto, empieza a estudiar la reciente enfermedad de Renfield (Pablo Álvarez Rubio), alguien que tiene devoción por la sangre y se alimenta de ratas y moscas. Es entonces cuando Van Helsing empieza a sospechar que Drácula es un vampiro y decide darle caza.
''Dracula's Daughter'' begins a few moments after ''Dracula'' ends. Count Dracula has just been destroyed by Abraham Van Helsing (Edward Van Sloan). Von Helsing is arrested by two Whitby policemen, Sergeant Wilkes (E. E. Clive) and constable Albert (Billy Bevan). Von Helsing is sent by the Whitby police to Scotland Yard, where he explains to Sir Basil Humphrey (Gilbert Emery) that he indeed did destroy Count Dracula, but because he had already been dead for over 500 years, it cannot be considered murder. Instead of hiring a lawyer, he enlists the aid of a psychiatrist, Dr. Jeffrey Garth (Otto Kruger), who was once one of his star students.
Hungarian Count Alucard (character) (Lon Chaney Jr.), a mysterious stranger, arrives in the U.S. invited by Katherine Caldwell (Louise Allbritton), one of the daughters of New Orleans plantation owner Colonel Caldwell (George Irving (American actor)). Shortly after his arrival, the Colonel dies of apparent heart failure and leaves his wealth to his two daughters, with Claire receiving all the money and Katherine his real estate "Dark Oaks." Katherine, a woman with a taste for the morbid, has been secretly dating Alucard and eventually marries him, shunning her long-time boyfriend Frank Stanley. Frank confronts the couple and tries to shoot Alucard, but the bullets pass through the Count's body and hit Katherine, seemingly killing her.
Count Dracula (Carradine) arrives at the castle home of Dr. Franz Edlemann (Onslow Stevens). The Count, who introduces himself as "Baron Latos", explains that he has come to Visaria to find a cure for his vampirism. Dr. Edlemann agrees to help. Together with his assistants, Milizia (Martha O'Driscoll) and the hunchbacked Nina (Poni Adams), he has been working on a mysterious plant, the clavaria formosa, whose spores have the ability to reshape bone. Edlemann explains that he thinks vampirism can be cured by a series of blood transfusions. Dracula agrees to this, and Edlemann uses his own blood for the transfusions. Dracula has his coffin placed in the castle basement.